Anti-H2AFY2/MACROH2A2 Antibody Picoband® A09931-1 from BosterBio

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Anti-H2AFY2/MACROH2A2 Antibody Picoband®

Western blot analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFY2/MACROH2A2 antigen affinity purified polyclonal antibody (Catalog # A09931-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFY2/MACROH2A2 at approximately 40 kDa. The expected band size for H2AFY2/MACROH2A2 is at 40 kDa.
Anti-H2AFY2/MACROH2A2 Antibody Picoband®

IHC analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1).
H2AFY2/MACROH2A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Anti-H2AFY2/MACROH2A2 Antibody Picoband®

IHC analysis of H2AFY2/MACROH2A2 using anti-H2AFY2/MACROH2A2 antibody (A09931-1).
H2AFY2/MACROH2A2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-H2AFY2/MACROH2A2 Antibody (A09931-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-H2AFY2/MACROH2A2 Antibody Picoband®

BosterBio's Anti-H2AFY2/MACROH2A2 Antibody Picoband® is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot. The Anti-H2AFY2/MACROH2A2 Antibody Picoband® was generated using macroH2A.2 histone, and MACROH2A2 as the antigen and it reacts with Homo Sapiens, Human, Mouse, and Rat.

Description

Boster Bio Anti-H2AFY2/MACROH2A2 Antibody Picoband™ catalog # A09931-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat

Anti-H2AFY2/MACROH2A2 Antibody Picoband®

Anti-H2AFY2/MACROH2A2 Antibody Picoband®

Anti-H2AFY2/MACROH2A2 Antibody Picoband®

Anti-H2AFY2/MACROH2A2 Antibody Picoband® from BosterBio

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Anti-H2AFY2/MACROH2A2 Antibody Picoband® from BosterBio

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